Description
Intended Uses: For the quantitative detection of Human Beta-1 adrenergic receptor (ADRB1) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ADRB1 in Human serum, plasma, tissue homogenates. An antibody specific for ADRB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADRB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB1 bound in the initial step. The color development is stopped and the intensity of the color is measured